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KMID : 0613820040140050794
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Journal of Life Science
2004 Volume.14 No. 5 p.794 ~ p.799
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Optimization of Cyclodextrin Glucanotransferase Immobilization on Amberlite IRA-900
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Seo Hyo-Jin
Jung Il-Hyong
Nam Soo-Wan
Kim Byung-Woo
Kim Sung-Koo
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Abstract
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Cyclodextrin glucanotransferase (CGTase) produced by Bacillus subtilis NA1/pKB1 was used for the production of cyclodextrin (CD). The enzyme was purified by ion exchange and gel filtration chromatography. The purified enzyme exhibited its maximum activity in the pH range of 6.0 to 7.0 and temperature range of 60 to 70℃. Immobilization of purified CGTase was carried out with various immobilization matrices. Amberlite IRA-900, a strong basic anion exchange resin, showed the highest immobilization ability (38 units per gram resin). Optimal pH and temperature for enzymatic reaction of the immobilized CGTase were pH 6.0 and 60℃. The activity of immobilized CGTase maintained more than a month and could be reused for a month in a continuous enzyme reactor for the production of CD.
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KEYWORD
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CGTase, Immobilization, Amberlite IRA-900, Cyclodextrin, Bacillus subtilis
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